77 research outputs found

    Tangible and Intangible Boundaries: The Case of Baoshan Port-City Interface in Shanghai

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    Instead of stressing that port cities are characterised by institutional fragmentations with many resulting conflicts, we claim that port cities might be highly constructive in terms of changing tangible and intangible boundaries. To capture this quality, we use the concept of ‘penumbral,’ a combination of perceptional aspects as well as tangible and intangible spatial constellations. This perspective is applied in the case of the Shanghai Baoshan port-city interface through the investigation of the changing tangible and intangible boundaries, and how planning relates to boundary changes in a context of spatial, industrial, and institutional multi-layered structures. Tangible refers to physical boundaries between the port and urban structure or district, while intangible refers to immaterial boundaries created by actors’ views on ports. Based on planning documents, direct observations, and 17 in-depth semi-structured interviews with local governments, port authority, planning departments, and companies, we find that one can indeed speak of penumbral boundaries, based on port-related values and ideas, and particularly on perceptions of the port and port businesses. Those perceptions are the initial power of changing and, following the idea of penumbral boundaries, blurring tangible and intangible boundaries. Finally, we suggest that, following the idea of penumbral boundaries, planning can play a stronger role in connecting the port and the city by first investigating how actors view the port and port businesses carefully, paying full attention to the specific relational context before formulating plans in the usual manner

    Governance of territorial and urban policies

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    ESPON project 2.3.2Governance of Territorial and Urban Policies from EU to Local Level holds an important position in the definition and elaboration of a common ground for investigating the institutional, instrumental ans procedural aspects of territorial and urban policies in Europe. The project focuses on the question how effective different systems are, e.g. considering a policy mix of spatial planning (in different forms implemented by Member States), local government powers and taxation policy in defining common spatial development strategies and objectives such as a polycentric urban system, balancing urban-rural needs, reviving derelict urban areas, urban regeneration, sustainable management of the natural and cultural assets. In that perspective, an analysis based on a comparative review of the instruments used, and stakeholders involved in various policy areas and processes, is being undertaken to draw some valuable conclusions of practical relevance on governance. The report sums up the main overall findings of the ESPON 2.3.2 project and presents in more detail the contributions delivered by IRPUD. The structure of this report is as follows. Part 1 summarizes the project in terms of research aims, hypotheses and key findings. The following parts present research work done by IRPUD. It starts with a German National Overview on the application of governance practices (part 2) and two case studies for urban and territorial governance (part 3). The second half of the report presents on a quantitative analysis of several indicators. Part 4 on data and indicators discusses data quality and develops the quantitative approach for measuring governance. In part 5 the report draws a synthesis of governance trends identified in the national case studies

    Application and effects of the ESDP in Member States

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    Between 2004 and 2006 IRPUD participated in two projects of the European Spatial Planning Observation Network, in short ESPON. Both projects fall into the policy impact studies of ESPON, trying to understand and identify the various effects of territorial policies implemented by the EU and its various bodies. The ESPON project 2.3.1 ??Application and Effects of the ESDP in Member States?? focused on the European Spatial Development Perspective and tried to analyse, which effects this bottom-up policy document finally generated when looking towards the planning systems of member states (and beyond). The ESPON project 2.3.2 Governance of Territorial and Urban Policies from the EU to Local Level on the one hand can be understood as a follow up of the mid-90ies Compendium of EU Planning Systems. On other hand, the study clearly goes beyond the earlier compendium trying to establish a deeper understanding of urban and territorial policies in Europe, not least surveying 29 states. IRPUD contributed to both projects various elements but in particular quantitative approaches towards the analysis. What needs to be stressed here is, that the quantitative approach in both cases constitutes only a very first attempt. Both policy fields are very complex analytical entities which do not lend easily for a quantitative indicator based survey. The results rather have to be seen as preliminary, raising many more questions than providing ready made answers. The reports presented here are excerpts of the final reports produced for ESPON. The complete versions can be found at www.espon.eu . The team for both projects (with varying responsibilities) consisted of Prof. Dr. Peter Ache, Alexandra Hill, Michael Höweler, Christian Lindner and Stefan Peters

    Space is luxury : selected proceedings of the 24th AESOP annual conference

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    AKT2/3 Subunits Render Guard Cell K+ Channels Ca2+ Sensitive

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    Inward-rectifying K+ channels serve as a major pathway for Ca2+-sensitive K+ influx into guard cells. Arabidopsis thaliana guard cell inward-rectifying K+ channels are assembled from multiple K+ channel subunits. Following the recent isolation and characterization of an akt2/3-1 knockout mutant, we examined whether the AKT2/3 subunit carries the Ca2+ sensitivity of the guard cell inward rectifier. Quantification of RT-PCR products showed that despite the absence of AKT2 transcripts in guard cells of the knockout plant, expression levels of the other K+ channel subunits (KAT1, KAT2, AKT1, and AtKC1) remained largely unaffected. Patch-clamp experiments with guard cell protoplasts from wild type and akt2/3-1 mutant, however, revealed pronounced differences in Ca2+ sensitivity of the K+ inward rectifier. Wild-type channels were blocked by extracellular Ca2+ in a concentration- and voltage-dependent manner. Akt2/3-1 mutants lacked the voltage-dependent Ca2+ block, characteristic for the K+ inward rectifier. To confirm the akt2/3-1 phenotype, two independent knockout mutants, akt2-1 and akt2::En-1 were tested, demonstrating that the loss of AKT2/3 indeed affects the Ca2+ dependence of guard cell inward rectifier. In contrast to AKT2 knockout plants, AKT1, AtKC1, and KAT1 loss-of-function mutants retained Ca2+ block of the guard cell inward rectifier. When expressed in HEK293 cells, AKT2 channel displayed a pronounced susceptibility toward extracellular Ca2+, while the dominant guard cell K+ channel KAT2 was Ca2+ insensitive. Thus, we conclude that the AKT2/3 subunit constitutes the Ca2+ sensitivity of the guard cell K+ uptake channel

    Educating planners in Europe: A review of 21st century study programmes

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    A  Education for urban, regional and spatial planning has become a regular subject throughout most European nations; this can be attributed in part to European policies promoting planning and spatially balanced development, but also to the recognition that planning can support sustainability. Nevertheless, there is lingering and justifiable concern about the status, profile and recognition of planning as a profession in its own right with the result that planning and planning education remain contested territories in academia. Conceptions of planning differ between countries and over time. The array of different planning cultures and associated educational models and pedagogies that traditionally have coexisted in Europe mean that education for planning can be either very visible or leading a shadow existence being embedded in programmes of other disciplines. While planning education provision customarily has been shaped by changes in planning practice paradigms and the profession, in 21st century Europe the provision is also influenced by European integration policies, the Bologna process and powerful transformations affecting the higher education sector writ large. This review seeks to advance our understanding of the complex dynamics at work, which to date have been only partially explored in the literature, by taking stock of the current state-of-play of planning education provision in Europe. Aside from examining the factors influencing planning education in Europe, an inventory of planning education programmes available throughout the member states of the Council of Europe was developed to quantify the provision as a critical first step. Figures indicate a substantial increase in the number of programmes when compared to limited historical data. Data also suggest an underdeveloped provision for education in planning in about ten per cent of European countries. Country case studies with historically differing planning cultures and education provision, i.e., Spain, Portugal, Finland, Poland, Slovakia, the United Kingdom and Switzerland are used to compare and explore trends and developments (e.g., in respect to programme structure, curriculum content and focus, professional conceptions, specialisms) in detail. Findings demonstrate, both, an enduring power of national preferences and traditions but also some emerging commonalities. Overall a picture of increasing pluralism and diversity of education models transpires in the aftermath of Bologna which may contravene efforts to establish cross-national professional recognition and standards. Education for planning seems to embrace trends to provide increasingly international learning experiences and degrees while the provision of flexible recognised (online) degree programmes remains sparse. Recommendations for future actions and strategies to further develop and strengthen the field which is at present complex and little coordinated conclude the contribution

    KDC1, a Novel Carrot Root Hair K+Channel CLONING, CHARACTERIZATION, AND EXPRESSION IN MAMMALIAN CELLS

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    Potassium is an essential nutrient which plays an important role in many aspects of plant growth and development. Plants have developed a number of highly specific mechanisms to take up potassium from the soil; these include the expression of K+ transporters and potassium channels in root cells. Despite the fact that root epidermal and hair cells are in direct contact with the soil, the role of these tissues in K+uptake is not well understood. Here we report the molecular cloning and functional characterization of a novel potassium channel KDC1 which forms part of a new subfamily of plant Kinchannels. Kdc1 was isolated from carrot root RNA andin situ hybridization experiments show Kdc1 to be highly expressed in root hair cells. Expressing the KDC1 protein in Chinese hamster ovary cells identified it as a voltage and pH-dependent inwardly rectifying potassium channel. An electrophysiological analysis of carrot root hair protoplasts confirmed the biophysical properties of the Kdc1 gene product (KDC1) in the heterologous expression system. KDC1 thus represents a major K+ uptake channel in carrot root hair cells

    Guard Cell-Specific Calcium Sensitivity of High Density and Activity SV/TPC1 Channels

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    The slow vacuolar (SV) channel, a Ca2+-regulated vacuolar cation conductance channel, in Arabidopsis thaliana is encoded by the single-copy gene AtTPC1. Although loss-of-function tpc1 mutants were reported to exhibit a stoma phenotype, knowledge about the underlying guard cell-specific features of SV/TPC1 channels is still lacking. Here we demonstrate that TPC1 transcripts and SV current density in guard cells were much more pronounced than in mesophyll cells. Furthermore, the SV channel in motor cells exhibited a higher cytosolic Ca2+ sensitivity than in mesophyll cells. These distinct features of the guard cell SV channel therefore probably account for the published stomatal phenotype of tpc1-

    Detecting early signs of heat and drought stress in Phoenix dactylifera (date palm)

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    Plants adapt to the environment by either long-term genome evolution or by acclimatization processes where the cellular processes and metabolism of the plant are adjusted within the existing potential in the genome. Here we studied the adaptation strategies in date palm, Phoenix dactylifera, under mild heat, drought and combined heat and drought by transcriptomic and metabolomic profiling. In transcriptomics data, combined heat and drought resembled heat response, whereas in metabolomics data it was more similar to drought. In both conditions, soluble carbohydrates, such as fucose, and glucose derivatives, were increased, suggesting a switch to carbohydrate metabolism and cell wall biogenesis. This result is consistent with the evidence from transcriptomics and cis-motif analysis. In addition, transcriptomics data showed transcriptional activation of genes related to reactive oxygen species in all three conditions (drought, heat, and combined heat and drought), suggesting increased activity of enzymatic antioxidant systems in cytosol, chloroplast and peroxisome. Finally, the genes that were differentially expressed in heat and combined heat and drought stresses were significantly enriched for circadian and diurnal rhythm motifs, suggesting new stress avoidance strategies.Peer reviewe
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